PDBe 1bi8

X-ray diffraction
2.8Å resolution

MECHANISM OF G1 CYCLIN DEPENDENT KINASE INHIBITION FROM THE STRUCTURES CDK6-P19INK4D INHIBITOR COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent kinase 6 Chains: A, C
Molecule details ›
Chains: A, C
Length: 326 amino acids
Theoretical weight: 36.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q00534 (Residues: 1-326; Coverage: 100%)
Gene names: CDK6, CDKN6
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-dependent kinase 4 inhibitor D Chains: B, D
Molecule details ›
Chains: B, D
Length: 166 amino acids
Theoretical weight: 17.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55273 (Residues: 1-166; Coverage: 100%)
Gene name: CDKN2D
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 96.86Å b: 116.06Å c: 131.49Å
α: 90° β: 110.63° γ: 90°
R-values:
R R work R free
0.255 0.255 0.308
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli