PDBe 1bi7

X-ray diffraction
3.4Å resolution

MECHANISM OF G1 CYCLIN DEPENDENT KINASE INHIBITION FROM THE STRUCTURE OF THE CDK6-P16INK4A TUMOR SUPPRESSOR COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent kinase 6 Chain: A
Molecule details ›
Chain: A
Length: 326 amino acids
Theoretical weight: 36.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q00534 (Residues: 1-326; Coverage: 100%)
Gene names: CDK6, CDKN6
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-dependent kinase inhibitor 2A Chain: B
Molecule details ›
Chain: B
Length: 156 amino acids
Theoretical weight: 16.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42771 (Residues: 1-156; Coverage: 100%)
  • Best match: P42771-2 (Residues: 1-105)
Gene names: CDKN2, CDKN2A, MTS1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P4122
Unit cell:
a: 123.1Å b: 123.1Å c: 112.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.33
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli