PDBe 1bf5

X-ray diffraction
2.9Å resolution

TYROSINE PHOSPHORYLATED STAT-1/DNA COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Signal transducer and activator of transcription 1-alpha/beta Chain: A
Molecule details ›
Chain: A
Length: 575 amino acids
Theoretical weight: 66.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42224 (Residues: 136-710; Coverage: 77%)
Gene name: STAT1
Sequence domains:
Structure domains:
DNA (5'-D(*AP*CP*AP*GP*TP*TP*TP*CP*CP*CP*GP*TP*AP*AP*AP*TP*G P*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 18 nucleotides
Theoretical weight: 5.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
DNA (5'-D(*TP*GP*CP*AP*TP*TP*TP*AP*CP*GP*GP*GP*AP*AP*AP*CP*T P*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.56 KDa
Source organism: Unidentified
Expression system: Not provided

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2221
Unit cell:
a: 76.6Å b: 148.2Å c: 181.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.293
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21
  • Not provided