PDBe 1bf2

X-ray diffraction
2Å resolution

STRUCTURE OF PSEUDOMONAS ISOAMYLASE

Released:
Source organism: Pseudomonas amyloderamosa
Primary publication:
Three-dimensional structure of Pseudomonas isoamylase at 2.2 A resolution.
J. Mol. Biol. 281 885-97 (1998)
PMID: 9719642

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoamylase Chain: A
Molecule details ›
Chain: A
Length: 750 amino acids
Theoretical weight: 81.14 KDa
Source organism: Pseudomonas amyloderamosa
UniProt:
  • Canonical: P10342 (Residues: 27-776; Coverage: 100%)
Gene name: iam
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 138.9Å b: 152.4Å c: 53.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.161 0.214