› 1bam

X-ray diffraction
1.95Å resolution

STRUCTURE OF RESTRICTION ENDONUCLEASE BAMHI PHASED AT 1.95 ANGSTROMS RESOLUTION BY MAD ANALYSIS

Released:
DOI: 10.2210/pdb1bam/pdb
PDB entry 1bam coloured by chain, front view.
PDB entry 1bam coloured by chain, side view.
PDB entry 1bam coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Structure of restriction endonuclease bamhi phased at 1.95 A resolution by MAD analysis.
Newman M, Strzelecka T, Dorner LF, Schildkraut I, Aggarwal AK
Structure 2 439-52 (1994)
PMID: 8081758

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme BamHI Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 24.6 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Not provided
UniProt:
  • Canonical: P23940 (Residues: 1-213; Coverage: 100%)
Gene name: bamHIR
Sequence domains: Restriction endonuclease BamHI
Structure domains: Restriction Endonuclease

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 76.4Å b: 46Å c: 69.4Å
α: 90° β: 110.5° γ: 90°
R-values:
R R work R free
0.19 0.19 not available
Expression system: Not provided

Quick links

Citations

1 review citation

Structure and function of restriction endonucleases.
Aggarwal AK. (1995)
32 other articles

8 mentions without citation

Structure and function of type II restriction endonucleases.
Pingoud et al. (2001)
7 more