PDBe 1ba3

X-ray diffraction
2.2Å resolution

FIREFLY LUCIFERASE IN COMPLEX WITH BROMOFORM

Released:

Function and Biology Details

Reaction catalysed:
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Luciferin 4-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 550 amino acids
Theoretical weight: 60.82 KDa
Source organism: Photinus pyralis
Expression system: Escherichia coli
UniProt:
  • Canonical: P08659 (Residues: 1-550; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P41212
Unit cell:
a: 119.56Å b: 119.56Å c: 95.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.239
Expression system: Escherichia coli