PDBe 1b9y

X-ray diffraction
3Å resolution

STRUCTURAL ANALYSIS OF PHOSDUCIN AND ITS PHOSPHORYLATION-REGULATED INTERACTION WITH TRANSDUCIN BETA-GAMMA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Phosducin Chain: C
Molecule details ›
Chain: C
Length: 246 amino acids
Theoretical weight: 28.16 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20942 (Residues: 1-246; Coverage: 100%)
Gene names: Pdc, Rpr1
Sequence domains: Phosducin
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(T) subunit gamma-T1 Chain: B
Molecule details ›
Chain: B
Length: 68 amino acids
Theoretical weight: 8.04 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02698 (Residues: 1-68; Coverage: 92%)
Gene name: GNGT1
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 76.47Å b: 89.92Å c: 102.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.266
Expression system: Escherichia coli BL21(DE3)