PDBe 1b9h

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF 3-AMINO-5-HYDROXYBENZOIC ACID (AHBA) SYNTHASE

Released:
Source organism: Amycolatopsis mediterranei
Primary publication:
Crystal structure of 3-amino-5-hydroxybenzoic acid (AHBA) synthase.
Biochemistry 38 9840-9 (1999)
PMID: 10433690

Function and Biology Details

Reaction catalysed:
5-amino-5-deoxy-3-dehydroshikimate = 3-amino-5-hydroxybenzoate + H(2)O. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-amino-5-hydroxybenzoate synthase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 42.26 KDa
Source organism: Amycolatopsis mediterranei
Expression system: Escherichia coli
UniProt:
  • Canonical: O52552 (Residues: 1-388; Coverage: 100%)
Gene names: AMES_0625, RAM_03205, rifD, rifK
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P3221
Unit cell:
a: 89.7Å b: 89.7Å c: 127.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.215 0.252
Expression system: Escherichia coli