PDBe 1b93

X-ray diffraction
1.9Å resolution

METHYLGLYOXAL SYNTHASE FROM ESCHERICHIA COLI

Released:
Source organism: Escherichia coli
Primary publication:
The crystal structure of methylglyoxal synthase from Escherichia coli.
Structure 7 309-17 (1999)
PMID: 10368300

Function and Biology Details

Reaction catalysed:
Glycerone phosphate = methylglyoxal + phosphate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylglyoxal synthase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 152 amino acids
Theoretical weight: 16.94 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A731 (Residues: 1-152; Coverage: 100%)
Gene names: JW5129, b0963, mgsA, yccG
Sequence domains: MGS-like domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6522
Unit cell:
a: 123.34Å b: 123.34Å c: 155.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 0.202
Expression system: Escherichia coli BL21(DE3)