PDBe 1b6g

X-ray diffraction
1.15Å resolution

HALOALKANE DEHALOGENASE AT PH 5.0 CONTAINING CHLORIDE

Released:
Source organism: Xanthobacter autotrophicus
Primary publication:
Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 refined at 1.15 A resolution.
Acta Crystallogr. D Biol. Crystallogr. 55 1273-90 (1999)
PMID: 10393294

Function and Biology Details

Reaction catalysed:
1-haloalkane + H(2)O = a primary alcohol + halide. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.57 KDa
Source organism: Xanthobacter autotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P22643 (Residues: 1-310; Coverage: 100%)
Gene name: dhlA
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21212
Unit cell:
a: 92.18Å b: 72.029Å c: 40.909Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.105 not available 0.145
Expression system: Escherichia coli