PDBe 1b5t

X-ray diffraction
2.5Å resolution

ESCHERICHIA COLI METHYLENETETRAHYDROFOLATE REDUCTASE

Released:

Function and Biology Details

Reaction catalysed:
5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5,10-methylenetetrahydrofolate reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 275 amino acids
Theoretical weight: 30.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEZ1 (Residues: 21-295; Coverage: 93%)
Gene names: JW3913, b3941, metF
Structure domains: TIM Barrel

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2
Unit cell:
a: 103Å b: 128.2Å c: 98.2Å
α: 90° β: 121.6° γ: 90°
R-values:
R R work R free
0.212 0.212 0.263
Expression system: Escherichia coli