PDBe 1b2f

X-ray diffraction
1.9Å resolution

PH AFFECTS GLU B13 SWITCHING AND SULFATE BINDING IN CUBIC INSULIN CRYSTALS (PH 6.98 COORDINATES)

Released:
Source organism: Sus scrofa
Primary publication:
Crystallographic titration of cubic insulin crystals: pH affects GluB13 switching and sulfate binding.
Acta Crystallogr. D Biol. Crystallogr. 59 670-6 (2003)
PMID: 12657786

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I213
Unit cell:
a: 78.774Å b: 78.774Å c: 78.774Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.244