PDBe 1b02

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THYMIDYLATE SYNTHASE A FROM BACILLUS SUBTILIS

Released:
Source organism: Bacillus subtilis

Function and Biology Details

Reaction catalysed:
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 32.85 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CI79 (Residues: 1-279; Coverage: 100%)
Gene names: BSU17680, thyA1
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate Synthase, chain A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 55.57Å b: 105.01Å c: 117.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.233
Expression system: Escherichia coli