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ECORV ENDONUCLEASE, UNLIGANDED, FORM B

X-ray diffraction
2.4Å resolution
PDB entry 1az3 coloured by chain, front view.
PDB entry 1az3 coloured by chain, side view.
PDB entry 1az3 coloured by chain, top view.
Source organism: Escherichia coli
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Type II restriction enzyme EcoRV
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Multimeric state: homo dimer
Accessible surface area: 19627.16 Å2
Buried surface area: 1891.49 Å2
Dissociation area: 945.75 Å2
Dissociation energy (ΔGdiss): 10.66 kcal/mol
Dissociation entropy (TΔSdiss): 12.8 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-137618

Macromolecules

Type II restriction enzyme EcoRV
Chains: A, B
Length: 244 amino acids
Theoretical weight: 28.56 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P04390 (Residues: 2-245; Coverage: 100%)
Gene name: ecoRVR
Pfam: Restriction endonuclease EcoRV
InterPro:
CATH: DNA mismatch repair MutH/Restriction endonuclease, type II
SCOP: Restriction endonuclease EcoRV
Type II restriction enzyme EcoRV in PDB entry 1az3, assembly 1, front view.
Type II restriction enzyme EcoRV in PDB entry 1az3, assembly 1, side view.
Type II restriction enzyme EcoRV in PDB entry 1az3, assembly 1, top view.
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