PDBe 1awc

X-ray diffraction
2.15Å resolution

MOUSE GABP ALPHA/BETA DOMAIN BOUND TO DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
GA-binding protein alpha chain Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 13.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00422 (Residues: 320-429; Coverage: 24%)
Gene names: E4tf1a, Gabpa
Sequence domains: Ets-domain
Structure domains: "winged helix" repressor DNA binding domain
GA-binding protein subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 153 amino acids
Theoretical weight: 16.83 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00420 (Residues: 5-157; Coverage: 40%)
Gene names: Gabpb, Gabpb1, Gabpb2
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
DNA (5'-D(*TP*TP*CP*CP*GP*GP*(BRU)P*GP*(BRU)P*AP*CP*TP*TP*CP*CP*GP*GP*TP*CP*AP*T)-3') Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.53 KDa
DNA (5'-D(*AP*AP*(BRU)P*GP*AP*CP*CP*GP*GP*AP*AP*GP*TP*AP*(CBR)P*AP*CP*(CBR)P*GP*GP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.71 KDa

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 201.6Å b: 34.55Å c: 59.26Å
α: 90° β: 99.83° γ: 90°
R-values:
R R work R free
0.211 0.211 0.282
Expression system: Escherichia coli BL21(DE3)