PDBe 1avz

X-ray diffraction
3Å resolution

V-1 NEF PROTEIN IN COMPLEX WITH WILD TYPE FYN SH3 DOMAIN

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C-terminal core protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 17.57 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03406 (Residues: 58-206; Coverage: 72%)
Gene name: nef
Structure domains: Nef Regulatory Factor
Tyrosine-protein kinase Fyn Chain: C
Molecule details ›
Chain: C
Length: 57 amino acids
Theoretical weight: 6.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06241 (Residues: 85-141; Coverage: 11%)
Gene name: FYN
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE D2AM
Spacegroup: P6522
Unit cell:
a: 108.2Å b: 108.2Å c: 223.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.27
Expression system: Escherichia coli BL21(DE3)