PDBe 1auo

X-ray diffraction
1.8Å resolution

CARBOXYLESTERASE FROM PSEUDOMONAS FLUORESCENS

Released:

Function and Biology Details

Reaction catalysed:
A carboxylic ester + H(2)O = an alcohol + a carboxylate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylesterase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 23.9 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53547 (Residues: 1-218; Coverage: 100%)
Gene name: estB
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P41212
Unit cell:
a: 82.04Å b: 82.04Å c: 145.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.273
Expression system: Escherichia coli