PDBe 1au7

X-ray diffraction
2.3Å resolution

PIT-1 MUTANT/DNA COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Pituitary-specific positive transcription factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 17 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P10037 (Residues: 128-273; Coverage: 50%)
Gene names: Ghf-1, Pit-1, Pit1, Pou1f1
Sequence domains: Homeobox domain
Structure domains:
CONSENSUS DNA 25-MER Chain: C
Molecule details ›
Chain: C
Length: 25 nucleotides
Theoretical weight: 7.72 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*TP*TP*CP*CP*TP*CP*AP*TP*GP*TP*AP*TP*AP*TP*AP*C P*AP*TP*GP*AP*GP* GP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.34 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P1
Unit cell:
a: 42.5Å b: 50.1Å c: 55.8Å
α: 76.7° β: 79.3° γ: 67.2°
R-values:
R R work R free
0.23 0.23 0.302
Expression systems:
  • Escherichia coli BL21
  • Not provided