PDBe 1ar1

X-ray diffraction
2.7Å resolution

Structure at 2.7 Angstrom Resolution of the Paracoccus Denitrificans two-subunit Cytochrome C Oxidase Complexed with an Antibody Fv Fragment

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Ig heavy chain V region 5-84 Chain: C
Molecule details ›
Chain: C
Length: 127 amino acids
Theoretical weight: 14.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18525 (Residues: 20-117; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig kappa chain V-V region MOPC 149 Chain: D
Molecule details ›
Chain: D
Length: 120 amino acids
Theoretical weight: 13.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01636 (Residues: 1-95, 96-108; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Cytochrome c oxidase subunit 1-beta Chain: A
Molecule details ›
Chain: A
Length: 558 amino acids
Theoretical weight: 62.49 KDa
Source organism: Paracoccus denitrificans
Expression system: Escherichia coli
UniProt:
  • Canonical: P98002 (Residues: 1-558; Coverage: 100%)
Gene name: ctaDII
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Structure domains: Cytochrome C Oxidase, chain A
Cytochrome c oxidase subunit 2 Chain: B
Molecule details ›
Chain: B
Length: 298 amino acids
Theoretical weight: 32.56 KDa
Source organism: Paracoccus denitrificans
Expression system: Escherichia coli
UniProt:
  • Canonical: P08306 (Residues: 1-298; Coverage: 100%)
Gene names: coiI, ctaB, ctaC
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 93.5Å b: 151Å c: 156.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.261
Expression system: Escherichia coli