Function and Biology

CRYSTAL STRUCTURE OF RECOMBINANT ASCORBATE PEROXIDASE

Source organism: Pisum sativum
Biochemical function: heme binding
Biological process: cellular oxidant detoxification
Cellular component: cytoplasm

EC 1.11.1.11: L-ascorbate peroxidase

Reaction catalysed:
2 L-ascorbate + H(2)O(2) + 2 H(+) = L-ascorbate + L-dehydroascorbate + 2 H(2)O.
Comments:
  • Oxidizes ascorbate and low molecular weight aromatic substrates.
  • The monodehydroascorbate radical produced is either directly reduced back to ascorbate by EC 1.6.5.4 or undergoes non-enzymatic disproportionation to ascorbate and dehydroascorbate.
Systematic name:
L-ascorbate:hydrogen-peroxide oxidoreductase
Alternative Name(s):
  • L-ascorbic acid peroxidase
  • L-ascorbic acid-specific peroxidase
  • Ascorbate peroxidase
  • Ascorbic acid peroxidase

Sequence family

Pfam Protein family (Pfam)
PF00141
Domain description: Peroxidase
Occurring in:
  1. L-ascorbate peroxidase, cytosolic
1 copy of Pfam domain PF00141 (Peroxidase) in L-ascorbate peroxidase, cytosolic in PDB 1apx.

InterPro InterPro annotations
IPR002207
Domain description: Class I peroxidase
Occurring in:
  1. L-ascorbate peroxidase, cytosolic
IPR002016
Domain description: Haem peroxidase, plant/fungal/bacterial
Occurring in:
  1. L-ascorbate peroxidase, cytosolic
IPR019793
Domain description: Peroxidases heam-ligand binding site
Occurring in:
  1. L-ascorbate peroxidase, cytosolic
IPR019794
Domain description: Peroxidase, active site
Occurring in:
  1. L-ascorbate peroxidase, cytosolic
IPR010255
Domain description: Haem peroxidase
Occurring in:
  1. L-ascorbate peroxidase, cytosolic

Structure domains

CATH CATH domains
1.10.520.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Peroxidase; domain 1
Homology: Peroxidase; domain 1
Occurring in:
  1. L-ascorbate peroxidase, cytosolic
1 copy of CATH domain 1.10.520.10 (Peroxidase; domain 1) in L-ascorbate peroxidase, cytosolic in PDB 1apx.
1.10.420.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Peroxidase; domain 2
Homology: Peroxidase, domain 2
Occurring in:
  1. L-ascorbate peroxidase, cytosolic
1 copy of CATH domain 1.10.420.10 (Peroxidase; domain 2) in L-ascorbate peroxidase, cytosolic in PDB 1apx.
SCOP SCOP annotation
48114
Class: All alpha proteins
Fold: Heme-dependent peroxidases
Superfamily: Heme-dependent peroxidases
Occurring in:
  1. L-ascorbate peroxidase, cytosolic
1 copy of SCOP domain 48114 (CCP-like) in L-ascorbate peroxidase, cytosolic in PDB 1apx.