PDBe 1apl

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF A MAT-ALPHA2 HOMEODOMAIN-OPERATOR COMPLEX SUGGESTS A GENERAL MODEL FOR HOMEODOMAIN-DNA INTERACTIONS

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mating-type protein ALPHA2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 83 amino acids
Theoretical weight: 9.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CY08 (Residues: 128-210; Coverage: 40%)
Gene names: ALPHA-2, MAT2A, MATAL2, MATALPHA2, YCR039C, YCR39C
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
DNA (5'-D(*AP*CP*AP*TP*GP*TP*AP*AP*TP*TP*CP*AP*TP*TP*TP*AP*C P*AP*CP*GP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 21 nucleotides
Theoretical weight: 6.38 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*GP*CP*GP*TP*GP*TP*AP*AP*AP*TP*GP*AP*AP*TP*TP*A P*CP*AP*TP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.5 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 60.78Å b: 39.93Å c: 68.79Å
α: 90° β: 97.35° γ: 90°
R-values:
R R work R free
0.226 0.226 not available
Expression systems:
  • Escherichia coli
  • Not provided