PDBe 1aot

Solution NMR

NMR STRUCTURE OF THE FYN SH2 DOMAIN COMPLEXED WITH A PHOSPHOTYROSYL PEPTIDE, MINIMIZED AVERAGE STRUCTURE

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Middle T antigen Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.47 KDa
Source organism: Mesocricetus auratus polyomavirus 1
UniProt:
  • Canonical: P03079 (Residues: 321-331; Coverage: 3%)
Tyrosine-protein kinase Fyn Chain: F
Molecule details ›
Chain: F
Length: 106 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06241 (Residues: 143-248; Coverage: 20%)
  • Best match: P06241-3 (Residues: 143-232)
Gene name: FYN
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Refinement method: DYNAMICAL SIMULATED ANNEALING
Expression system: Escherichia coli BL21(DE3)