PDBe 1amu

X-ray diffraction
1.9Å resolution

PHENYLALANINE ACTIVATING DOMAIN OF GRAMICIDIN SYNTHETASE 1 IN A COMPLEX WITH AMP AND PHENYLALANINE

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-phenylalanine + H(2)O = AMP + diphosphate + D-phenylalanine. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gramicidin S synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 563 amino acids
Theoretical weight: 64.13 KDa
Source organism: Brevibacillus brevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C061 (Residues: 1-556; Coverage: 51%)
Gene names: grs1, grsA
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 61.68Å b: 154.77Å c: 65.3Å
α: 90° β: 93.91° γ: 90°
R-values:
R R work R free
0.213 0.213 0.246
Expression system: Escherichia coli