› 1ame

X-ray diffraction
1.65Å resolution

CRYSTAL STRUCTURE OF TYPE III ANTIFREEZE PROTEIN AT 4 C

Released:
DOI: 10.2210/pdb1ame/pdb
PDB entry 1ame coloured by chain, front view.
PDB entry 1ame coloured by chain, side view.
PDB entry 1ame coloured by chain, top view.
Source organism: Zoarces americanus
Primary publication:
Structure of type III antifreeze protein at 277 K.
Ye Q, Leinala E, Jia Z
Acta Crystallogr D Biol Crystallogr 54 700-2 (1998)
PMID: 9761880

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-3 ice-structuring protein HPLC 12 Chain: A
Molecule details ›
Chain: A
Length: 67 amino acids
Theoretical weight: 7.05 KDa
Source organism: Zoarces americanus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19614 (Residues: 1-63; Coverage: 96%)
Structure domains: Antifreeze-like/N-acetylneuraminic acid synthase C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 32.69Å b: 38.84Å c: 47.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.282
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Properties, potentials, and prospects of antifreeze proteins.
Venketesh et al. (2008)
1 more

8 mentions without citation

Thoroughly sampling sequence space: large-scale protein design of structural ensembles.
Larson et al. (2002)
7 more