PDBe 1am9

X-ray diffraction
2.3Å resolution

HUMAN SREBP-1A BOUND TO LDL RECEPTOR PROMOTER

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Processed sterol regulatory element-binding protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 82 amino acids
Theoretical weight: 9.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P36956 (Residues: 319-400; Coverage: 7%)
Gene names: BHLHD1, SREBF1, SREBP1
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: HLH, helix-loop-helix DNA-binding domain
DNA (5'-D(*TP*TP*GP*CP*AP*GP*TP*GP*GP*GP*GP*TP*GP*AP*TP*CP*T )-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 17 nucleotides
Theoretical weight: 5.29 KDa
Source organism: Synthetic construct
Expression system: Not provided
DNA (5'-D(*CP*AP*TP*GP*AP*GP*AP*TP*CP*AP*CP*CP*CP*CP*AP*CP*T P*GP*CP*AP*A)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 21 nucleotides
Theoretical weight: 6.36 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6122
Unit cell:
a: 94.63Å b: 94.63Å c: 459.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.219 0.278
Expression systems:
  • Escherichia coli
  • Not provided