PDBe 1ako

X-ray diffraction
1.7Å resolution

EXONUCLEASE III FROM ESCHERICHIA COLI

Released:

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exodeoxyribonuclease III Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 31.01 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09030 (Residues: 1-268; Coverage: 100%)
Gene names: JW1738, b1749, xth, xthA
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Deoxyribonuclease I; Chain A

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P3121
Unit cell:
a: 107.8Å b: 107.8Å c: 42.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.169 0.202
Expression system: Escherichia coli BL21(DE3)