PDBe 1afr

X-ray diffraction
2.4Å resolution

STEAROYL-ACYL CARRIER PROTEIN DESATURASE FROM CASTOR SEEDS

Released:

Function and Biology Details

Reaction catalysed:
Stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O(2) + 2 H(+) = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 345 amino acids
Theoretical weight: 39.75 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P22337 (Residues: 52-396; Coverage: 87%)
Sequence domains: Fatty acid desaturase
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 82.2Å b: 147.8Å c: 198.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.285
Expression system: Escherichia coli