› 1ad3

X-ray diffraction
2.6Å resolution

CLASS 3 ALDEHYDE DEHYDROGENASE COMPLEX WITH NICOTINAMIDE-ADENINE-DINUCLEOTIDE

Released:
DOI: 10.2210/pdb1ad3/pdb
PDB entry 1ad3 coloured by chain, front view.
PDB entry 1ad3 coloured by chain, side view.
PDB entry 1ad3 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The first structure of an aldehyde dehydrogenase reveals novel interactions between NAD and the Rossmann fold.
Liu ZJ, Sun YJ, Rose J, Chung YJ, Hsiao CD, Chang WR, Kuo I, Perozich J, Lindahl R, Hempel J, Wang BC
Nat Struct Biol 4 317-26 (1997)
PMID: 9095201

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(P)(+) + H(2)O = a carboxylate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, dimeric NADP-preferring Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 50.31 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P11883 (Residues: 3-453; Coverage: 100%)
Gene names: Aldd, Aldh3, Aldh3a1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 64.95Å b: 170.95Å c: 47.16Å
α: 90° β: 110.25° γ: 90°
R-values:
R R work R free
0.177 0.177 0.279
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily.
Marchitti et al. (2008)
18 more

16 mentions without citation

Sequence-structure analysis of FAD-containing proteins.
Dym et al. (2001)
15 more