PDBe 1a9x

X-ray diffraction
1.8Å resolution

CARBAMOYL PHOSPHATE SYNTHETASE: CAUGHT IN THE ACT OF GLUTAMINE HYDROLYSIS

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero octamer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carbamoyl-phosphate synthase large chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 1073 amino acids
Theoretical weight: 117.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00968 (Residues: 1-1073; Coverage: 100%)
Gene names: JW0031, b0033, carB, pyrA
Sequence domains:
Structure domains:
Carbamoyl-phosphate synthase small chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 379 amino acids
Theoretical weight: 41.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6F1 (Residues: 2-380; Coverage: 99%)
Gene names: JW0030, b0032, carA, pyrA
Sequence domains:
Structure domains:

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 152.1Å b: 164.4Å c: 332.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 not available not available
Expression system: Escherichia coli