PDBe 1a79

X-ray diffraction
2.28Å resolution

CRYSTAL STRUCTURE OF THE TRNA SPLICING ENDONUCLEASE FROM METHANOCOCCUS JANNASCHII

Released:
Primary publication:
Crystal structure and evolution of a transfer RNA splicing enzyme.
Science 280 279-84 (1998)
PMID: 9535656

Function and Biology Details

Reaction catalysed:
PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. 
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA-splicing endonuclease Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 171 amino acids
Theoretical weight: 19.75 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58819 (Residues: 9-179; Coverage: 96%)
Gene names: MJ1424, endA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 61.95Å b: 80.04Å c: 193.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.267
Expression system: Escherichia coli