PDBe 1a69

X-ray diffraction
2.1Å resolution

PURINE NUCLEOSIDE PHOSPHORYLASE IN COMPLEX WITH FORMYCIN B AND SULPHATE (PHOSPHATE)

Released:

Function and Biology Details

Reaction catalysed:
(1) Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. (2) Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase DeoD-type Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 238 amino acids
Theoretical weight: 25.85 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ABP8 (Residues: 2-239; Coverage: 100%)
Gene names: JW4347, b4384, deoD, pup
Sequence domains: Phosphorylase superfamily
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P6122
Unit cell:
a: 123.11Å b: 123.11Å c: 241.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 not available not available