› 1a5i

X-ray diffraction
2.9Å resolution

CATALYTIC DOMAIN OF VAMPIRE BAT (DESMODUS ROTUNDUS) SALIVA PLASMINOGEN ACTIVATOR IN COMPLEX WITH EGR-CMK (GLU-GLY-ARG CHLOROMETHYL KETONE)

Released:
DOI: 10.2210/pdb1a5i/pdb
PDB entry 1a5i coloured by chain, front view.
PDB entry 1a5i coloured by chain, side view.
PDB entry 1a5i coloured by chain, top view.
Source organism: Desmodus rotundus
Primary publication:
Catalytic domain structure of vampire bat plasminogen activator: a molecular paradigm for proteolysis without activation cleavage.
Renatus M, Stubbs MT, Huber R, Bringmann P, Donner P, Schleuning WD, Bode W
Biochemistry 36 13483-93 (1997)
PMID: 9354616

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Salivary plasminogen activator alpha 1 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.72 KDa
Source organism: Desmodus rotundus
Expression system: Mesocricetus auratus
UniProt:
  • Canonical: P98119 (Residues: 213-477; Coverage: 60%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand 0GJ 1 x 0GJ
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 73.86Å b: 73.86Å c: 135.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 not available
Expression system: Mesocricetus auratus

Quick links

Citations

10 review citations

Structural and energetic determinants of the S1-site specificity in serine proteases.
Czapinska et al. (1999)
9 more

6 mentions without citation

Structural Biology and Protein Engineering of Thrombolytics.
Mican et al. (2019)
5 more