PDBe 1a4i

X-ray diffraction
1.5Å resolution

HUMAN TETRAHYDROFOLATE DEHYDROGENASE / CYCLOHYDROLASE

Released:

Function and Biology Details

Reactions catalysed:
5,10-methylenetetrahydrofolate + NADP(+) = 5,10- methenyltetrahydrofolate + NADPH. 
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. 
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 32.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11586 (Residues: 1-301; Coverage: 32%)
Gene names: MTHFC, MTHFD, MTHFD1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 67.523Å b: 135.843Å c: 61.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.235
Expression system: Escherichia coli