PDBe 1a14

X-ray diffraction
2.5Å resolution

COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Neuraminidase Chain: N
Molecule details ›
Chain: N
Length: 388 amino acids
Theoretical weight: 43.72 KDa
Source organism: Influenza A virus
UniProt:
  • Canonical: P03472 (Residues: 83-470; Coverage: 83%)
Gene name: NA
Structure domains: Neuraminidase
Ig heavy chain V region 3 Chain: H
Molecule details ›
Chain: H
Length: 120 amino acids
Theoretical weight: 13.23 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01749 (Residues: 20-117; Coverage: 100%)
Gene names: Igh-VJ558, Ighv1-61
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig kappa chain V-V region HP 93G7 Chain: L
Molecule details ›
Chain: L
Length: 104 amino acids
Theoretical weight: 11.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01645 (Residues: 1-102; Coverage: 94%)
Structure domains: Immunoglobulins

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: I422
Unit cell:
a: 165.3Å b: 165.3Å c: 182.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.27
Expression system: Escherichia coli