PDBe 1a0i

X-ray diffraction
2.6Å resolution

ATP-DEPENDENT DNA LIGASE FROM BACTERIOPHAGE T7 COMPLEX WITH ATP

Released:

Function and Biology Details

Reaction catalysed:
ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.89 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P00969 (Residues: 2-349; Coverage: 97%)
Gene name: 1.3
Sequence domains: ATP dependent DNA ligase domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P21212
Unit cell:
a: 64.7Å b: 85.2Å c: 79.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.341
Expression system: Escherichia coli