› 1a09

X-ray diffraction
2Å resolution

C-src (SH2 domain) complexed with ace-formyl phosphotyr-glu-(n,n-dipentyl amine)

Released:
DOI: 10.2210/pdb1a09/pdb
PDB entry 1a09 coloured by chain, front view.
PDB entry 1a09 coloured by chain, side view.
PDB entry 1a09 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Peptide ligands of pp60(c-src) SH2 domains: a thermodynamic and structural study.
Charifson PS, Shewchuk LM, Rocque W, Hummel CW, Jordan SR, Mohr C, Pacofsky GJ, Peel MR, Rodriguez M, Sternbach DD, Consler TG
Biochemistry 36 6283-93 (1997)
PMID: 9174343

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146382 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P12931 (Residues: 144-249; Coverage: 20%)
Gene names: SRC, SRC1
Sequence domains: SH2 domain
Structure domains: SH2 domain
ACE-FORMYL PHOSPHOTYR-GLU-(N,N-DIPENTYL AMINE) Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 amino acids
Theoretical weight: 586 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTH 2 x PTH

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 51.9Å b: 66Å c: 74.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 not available
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

6 review citations

Computer simulations with explicit solvent: recent progress in the thermodynamic decomposition of free energies and in modeling electrostatic effects.
Levy et al. (1998)
5 more

5 mentions without citation

Reconstruction of ancestral protein sequences and its applications.
Cai et al. (2004)
4 more