PDBe 1a05

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX OF 3-ISOPROPYLMALATE DEHYDROGENASE FROM THIOBACILLUS FERROOXIDANS WITH 3-ISOPROPYLMALATE

Released:

Function and Biology Details

Reaction catalysed:
(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH. 

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-isopropylmalate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 38.51 KDa
Source organism: Acidithiobacillus ferrooxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56268 (Residues: 1-358; Coverage: 100%)
Gene name: leuB
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-C
Spacegroup: P21212
Unit cell:
a: 56.54Å b: 114.24Å c: 130.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.275
Expression system: Escherichia coli