PDBe 1a02

X-ray diffraction
2.7Å resolution

STRUCTURE OF THE DNA BINDING DOMAINS OF NFAT, FOS AND JUN BOUND TO DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Proto-oncogene c-Fos Chain: F
Molecule details ›
Chain: F
Length: 56 amino acids
Theoretical weight: 6.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01100 (Residues: 138-193; Coverage: 15%)
Gene names: FOS, G0S7
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Transcription factor AP-1 Chain: J
Molecule details ›
Chain: J
Length: 56 amino acids
Theoretical weight: 6.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05412 (Residues: 253-308; Coverage: 17%)
Gene name: JUN
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Nuclear factor of activated T-cells, cytoplasmic 2 Chain: N
Molecule details ›
Chain: N
Length: 301 amino acids
Theoretical weight: 34.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13469 (Residues: 392-678; Coverage: 31%)
Gene names: NFAT1, NFATC2, NFATP
Sequence domains: Rel homology DNA-binding domain
Structure domains:
DNA (5'-D(*DAP*DAP*DCP*DTP*DAP*DTP*DGP*DAP*DAP*DAP*DCP*DAP*DAP*DAP*DTP*DTP*DTP*DTP*DCP*DC)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.09 KDa
DNA (5'-D(*DTP*DTP*DGP*DGP*DAP*DAP*DAP*DAP*DTP*DTP*DTP*DGP*DTP*DTP*DTP*DCP*DAP*DTP*DAP*DG)-3') Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.18 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 64.66Å b: 85.46Å c: 83.37Å
α: 90° β: 112.03° γ: 90°
R-values:
R R work R free
0.246 0.246 0.303
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)