PDBe 1zs4

X-ray diffraction
1.7Å resolution

Structure of bacteriophage lambda cII protein in complex with DNA

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional activator II Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 83 amino acids
Theoretical weight: 9.32 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03042 (Residues: 4-82; Coverage: 81%)
Gene names: cII, lambdap59
Structure domains: lambda repressor-like DNA-binding domains
DNA - 27mer Chain: U
Molecule details ›
Chain: U
Length: 27 nucleotides
Theoretical weight: 8.26 KDa
Source organism: Escherichia virus Lambda
Expression system: Not provided
DNA - 27mer Chain: T
Molecule details ›
Chain: T
Length: 27 nucleotides
Theoretical weight: 8.32 KDa
Source organism: Escherichia virus Lambda
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P21
Unit cell:
a: 44.771Å b: 97.132Å c: 59.869Å
α: 90° β: 105.36° γ: 90°
R-values:
R R work R free
0.209 0.209 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided