PDBe 1zpt

X-ray diffraction
1.95Å resolution

Escherichia coli Methylenetetrahydrofolate Reductase (reduced) complexed with NADH, pH 7.25


Function and Biology Details

Reaction catalysed:
5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
5,10-methylenetetrahydrofolate reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 304 amino acids
Theoretical weight: 34.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P0AEZ1 (Residues: 1-296; Coverage: 100%)
Gene names: JW3913, b3941, metF
Sequence domains: Methylenetetrahydrofolate reductase
Structure domains: TIM Barrel

Ligands and Environments

Cofactor: Ligand NAI 3 x NAI

Cofactor: Ligand FAD 3 x FAD
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 103.613Å b: 127.735Å c: 97.831Å
α: 90° β: 121.73° γ: 90°
R R work R free
0.219 0.219 0.246
Expression system: Escherichia coli