PDBe 1zi0

X-ray diffraction
2.6Å resolution

A Superhelical Spiral in Escherichia coli DNA Gyrase A C-terminal Domain Imparts Unidirectional Supercoiling Bias

Released:

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit A Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AES4 (Residues: 535-841; Coverage: 35%)
Gene names: JW2225, b2231, gyrA, hisW, nalA, parD
Sequence domains: DNA gyrase C-terminal domain, beta-propeller
Structure domains: DNA gyrase/topoisomerase IV, subunit A, C-terminal

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1, NSLS BEAMLINE X4A
Spacegroup: I222
Unit cell:
a: 104.216Å b: 121.432Å c: 177.957Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.266
Expression system: Escherichia coli