1yy9

X-ray diffraction
2.6Å resolution

Structure of the extracellular domain of the epidermal growth factor receptor in complex with the Fab fragment of cetuximab/Erbitux/IMC-C225

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-208212 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Epidermal growth factor receptor Chain: A
Molecule details ›
Chain: A
Length: 624 amino acids
Theoretical weight: 69.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 25-642; Coverage: 52%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains:
Structure domains:
Cetuximab Fab Light chain Chain: C
Molecule details ›
Chain: C
Length: 213 amino acids
Theoretical weight: 23.29 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Cetuximab Fab Heavy chain Chain: D
Molecule details ›
Chain: D
Length: 221 amino acids
Theoretical weight: 23.73 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 77.823Å b: 70.861Å c: 147.122Å
α: 90° β: 102.48° γ: 90°
R-values:
R R work R free
0.242 0.239 0.289
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus