PDBe 1yrw

X-ray diffraction
1.7Å resolution

Crystal Structure of E.coli ArnA Transformylase Domain

Released:

Function and Biology Details

Reactions catalysed:
UDP-alpha-D-glucuronate + NAD(+) = UDP-beta-L-threo-pentapyranos-4-ulose + CO(2) + NADH. 
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinose. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional polymyxin resistance protein ArnA Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 32.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77398 (Residues: 1-300; Coverage: 46%)
Gene names: JW2249, arnA, b2255, pmrI, yfbG
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 59.103Å b: 113.253Å c: 124.946Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.225
Expression system: Escherichia coli