PDBe 1ym4

X-ray diffraction
2.25Å resolution

Function and Biology Details

Reaction catalysed:
Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu- Phe in the Swedish variant of Alzheimer's amyloid precursor protein. 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-secretase 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 408 amino acids
Theoretical weight: 45.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P56817 (Residues: 48-453; Coverage: 85%)
Gene names: BACE, BACE1, KIAA1149
Sequence domains: Eukaryotic aspartyl protease
Structure domains: Acid Proteases
NVP-AMK640 INHIBITOR Chains: X, Y, Z
Molecule details ›
Chains: X, Y, Z
Length: 5 amino acids
Theoretical weight: 643 Da
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 223.678Å b: 107.751Å c: 61.475Å
α: 90° β: 100.32° γ: 90°
R-values:
R R work R free
0.245 0.24 0.288
Expression systems:
  • Escherichia coli
  • Not provided