PDBe 1yjd

X-ray diffraction
2.7Å resolution

Crystal structure of human CD28 in complex with the Fab fragment of a mitogenic antibody (5.11A1)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab fragment of 5.11A1 antibody light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.39 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab fragment of 5.11A1 antibody heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 24.11 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
T-cell-specific surface glycoprotein CD28 Chain: C
Molecule details ›
Chain: C
Length: 140 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P10747 (Residues: 17-152; Coverage: 66%)
Gene name: CD28
Sequence domains: ICOS V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: C2
Unit cell:
a: 191.219Å b: 47.417Å c: 71.842Å
α: 90° β: 94.45° γ: 90°
R-values:
R R work R free
0.239 0.239 0.282
Expression system: Cricetulus griseus