PDBe 1ycq

X-ray diffraction
2.3Å resolution

XENOPUS LAEVIS MDM2 BOUND TO THE TRANSACTIVATION DOMAIN OF HUMAN P53

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.17 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P56273 (Residues: 13-119; Coverage: 23%)
Gene name: mdm2
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain
Cellular tumor antigen p53 Chain: B
Molecule details ›
Chain: B
Length: 17 amino acids
Theoretical weight: 2.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04637 (Residues: 13-29; Coverage: 4%)
Gene names: P53, TP53

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6322
Unit cell:
a: 84.96Å b: 84.96Å c: 63.89Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.188 0.253
Expression systems:
  • Escherichia coli
  • Not provided