PDBe 1y28

X-ray diffraction
2.1Å resolution

Crystal structure of the R220A metBJFIXL HEME domain

Released:

Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor protein FixL Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.83 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P23222 (Residues: 141-270; Coverage: 26%)
Gene names: bll2760, fixL
Sequence domains: PAS fold
Structure domains: Beta-Lactamase

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: R32
Unit cell:
a: 126.761Å b: 126.761Å c: 58.056Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.251 0.287
Expression system: Escherichia coli