› 1xwi

X-ray diffraction
2.8Å resolution

Crystal Structure of VPS4B

Released:
DOI: 10.2210/pdb1xwi/pdb
PDB entry 1xwi coloured by chain, front view.
PDB entry 1xwi coloured by chain, side view.
PDB entry 1xwi coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and mechanistic studies of VPS4 proteins.
Scott A, Chung HY, Gonciarz-Swiatek M, Hill GC, Whitby FG, Gaspar J, Holton JM, Viswanathan R, Ghaffarian S, Hill CP, Sundquist WI
EMBO J 24 3658-69 (2005)
PMID: 16193069

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 4B Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 35.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75351 (Residues: 123-444; Coverage: 73%)
Gene names: MIG1, SKD1, VPS42, VPS4B
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P65
Unit cell:
a: 88.058Å b: 88.058Å c: 112.574Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.192 0.26
Expression system: Escherichia coli

Quick links

Citations

47 review citations

The ESCRT pathway.
Henne et al. (2011)
46 more

13 mentions without citation

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
12 more