› 1xtp

X-ray diffraction
1.94Å resolution

Structural Analysis of Leishmania major LMAJ004091AAA, a SAM-dependent methyltransferase of the DUF858/Pfam05891 family

Released:
DOI: 10.2210/pdb1xtp/pdb
PDB entry 1xtp coloured by chain, front view.
PDB entry 1xtp coloured by chain, side view.
PDB entry 1xtp coloured by chain, top view.
Source organism: Leishmania major strain Friedlin
Entry authors: Robien MA, Hol WGJ, Structural Genomics of Pathogenic Protozoa Consortium (SGPP)

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha N-terminal protein methyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.82 KDa
Source organism: Leishmania major strain Friedlin
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4Q7M2 (Residues: 1-250; Coverage: 100%)
Gene name: LMJF_30_0810
Sequence domains: AdoMet dependent proline di-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAI 1 x SAI
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 40.208Å b: 42.56Å c: 69.853Å
α: 90° β: 98.27° γ: 90°
R-values:
R R work R free
0.157 0.153 0.219
Expression system: Escherichia coli

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