PDBe 1xrj

X-ray diffraction
2Å resolution

Rapid structure determination of human uridine-cytidine kinase 2 using a conventional laboratory X-ray source and a single samarium derivative

Released:

Function and Biology Details

Reaction catalysed:
ATP + uridine = ADP + UMP
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine-cytidine kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 29.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9BZX2 (Residues: 1-261; Coverage: 100%)
Gene names: UCK2, UMPK
Sequence domains: Phosphoribulokinase / Uridine kinase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 88.63Å b: 109.32Å c: 64.52Å
α: 90° β: 94.96° γ: 90°
R-values:
R R work R free
0.194 0.194 0.219
Expression system: Escherichia coli BL21